Comparative Investigation on Formation and Accumulation of Rare Phenylpropanoids in Plants and in vitro Cultures of Pimpinella major
نویسندگان
چکیده
Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropa noids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was com pared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoids. In comparison, the leaf/root-differentiating callus culture promoted the accumulation of epoxy-pseudoisoeugenol tiglate (up to 90 mg/100 g fr.wt.) but not that of anol-derivatives. The accumulated amount of EPT in PMD-SH was comparable with that in plant seedlings.
منابع مشابه
Production and Accumulation of Phenylpropanoids in Tissue and Organ Cultures of Pimpinella anisum
Recently we reported the comparative studies on the production and accumulation of phenylpropanoids in plants and in cell cultures of anise [1, 2]. It was shown that cell cultures did not produce anethole, the main constituent in the fruit oil of anise, but instead regularly epoxy-pseudoisoeugenol(2-methylbutyrate). We first identified this unusually substituted phenylpropanoid in the root of P...
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